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目的探讨不同类型免疫反应在实验性自身免疫性脑脊髓炎(EAE)发病中的作用。方法 24只SD大鼠随机均分为EAE组和对照组,通过行为观测和大脑微观形态学确认免疫诱导的EAE模型,用ELISA法检测淋巴结和脾细胞培养上清液中IL-4、IFN-γ和血清中IgG水平,流式细胞术检测淋巴结和脾细胞中IL-17及Foxp3细胞频数。结果与对照组相比,EAE组IgG、IFN-γ、IL-17及IL-4水平均明显增高(P<0.05或P<0.01)。结论 Th1、Th2和Th17免疫细胞在EAE的发病均起着重要的作用。
Objective To investigate the role of different types of immune responses in the pathogenesis of experimental autoimmune encephalomyelitis (EAE). Methods Twenty-four SD rats were randomly divided into EAE group and control group. The immune-induced EAE model was confirmed by behavioral observation and morphological observation of the brain, and the levels of IL-4 and IFN- γ and serum IgG levels, flow cytometry lymph node and spleen cells IL-17 and Foxp3 cell frequency. Results Compared with the control group, the levels of IgG, IFN-γ, IL-17 and IL-4 in EAE group were significantly increased (P <0.05 or P <0.01). Conclusion Th1, Th2 and Th17 immune cells play an important role in the pathogenesis of EAE.