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目的： 观察海南省与云南省两地微小按蚊之间是否存在种间差异。方法： 在两地牛房采集微小按蚊，单雌驯养繁殖， 用强迫交配方法进行杂交试验， 观察 Ｆ１ 代的可育性。制作杂种 Ｆ１ 卵巢营养细胞多线染色体标本， 观察染色体各区域的联会情况。结果： 云南省微小按蚊（ Ｙ） ♀×海南省微小按蚊（ Ｈ） ♂杂交组卵孵化率为０ ， 卵内无胚胎形成。（ Ｈ♀× Ｙ♂） Ｆ１ 各回交组中， 大多数卵孵化率显著低于亲本。（ Ｈ♀× Ｙ♂） Ｆ１ 雌蚊卵巢营养细胞多线染色体３ Ｒ 的２９ 区、３６ 区及３７ 区， Ｘ 性染色体的４ 区和６ 区出现恒定不联会。结论： 海南省与云南省两地微小按蚊已出现明显生殖隔离， 系两个不同的亲缘种。
Objective: To observe whether there is interspecific difference between Anopheles minimus in Hainan Province and Yunnan Province. Methods: Anopheles minimus was collected in the bovine australia, and domesticated and domesticated single hermaphrodites. The forced mating method was used to test the fertility of F1 generation. Multi-line chromosome samples of hybrid F1 ovarian vegetative cells were made to observe the association of chromosomes in various regions. Results: The hatching rate of egg hatching in Anopheles minor mosquitoes (Y) ♀ × Hainan Anopheles minitus (H) ♂ was 0, but there was no embryo in eggs. (H♀ × Y♂) F1, the hatching rate of most eggs was significantly lower than that of the parents. (H♀ × Y♂) F1 female mosquito ovarian vegetative cell line 3 R 29, 36 and 37 districts, X sex chromosomes 4 and 6 areas appear not to associate. Conclusion: Anopheles minimus in both Hainan and Yunnan Provinces have shown obvious reproductive isolation, which is related to two different relatives.