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目的:研究抗纤软肝颗粒(KXR)对血小板源生长因子(PDGF)诱导的肝星状细胞(HSC)增殖的影响。方法:采用无血清培养,不同浓度的KXR温育HSC 24 h后,PDGF-BB(10 ng／ml)刺激24 h,再加入上述浓度的KXR 3 h后,又加入PDGF-BB(10 ng／ml)作用 5 min,然后收集细胞。采用细胞计数法及流式细胞仪测定细胞增殖及细胞周期。结果:无血清培养显示该方对于PDGF诱导的细胞增殖具有抑制作用,并呈剂量依赖性(P<0.01)。流式细胞术分析提示KXR能抑制PDGF诱导的HSC DNA合成期及合成后期和分裂期DNA百分含量,阻断细胞由静止期／DNA合成前期向DNA合成期转化,呈剂量依赖性(5 mg／ml组作用最显著,P<0.01).结论:KXR能抑制PDGF诱导的HSC增殖。
Objective: To study the effect of anti-fibrosis liver granules (KXR) on the proliferation of platelet-derived growth factor (PDGF)-induced hepatic stellate cells (HSCs). Methods: After serum-free culture, different concentrations of KXR incubated HSC for 24 h, PDGF-BB (10 ng/ml) stimulated for 24 h, and after adding the above concentration of KXR for 3 h, PDGF-BB was added (10 ng/ml). Ml) For 5 min, collect the cells. Cell proliferation and cell cycle were measured using cell counting and flow cytometry. RESULTS: Serum-free culture showed that this side had an inhibitory effect on PDGF-induced cell proliferation in a dose-dependent manner (P<0.01). Flow cytometry analysis suggested that KXR could inhibit PDGF-induced HSC DNA synthesis and the percentage of DNA in late synthesis and division, blocking cells from quiescent/DNA synthesis to DNA synthesis, in a dose-dependent manner (5 mg The most significant effect was in the /ml group (P<0.01). Conclusion: KXR can inhibit PDGF-induced HSC proliferation.