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目的:合成木犀草素-Cr(Ⅲ),考察木犀草素及木犀草素-Cr(Ⅲ)对α-葡萄糖苷酶的抑制作用,确定抑制类型。方法:在无水乙醇中,用木犀草素与醋酸铬搅拌合成木犀草素-Cr(Ⅲ)配合物;用IR、UV、TGDTA结合元素分析对结构进行表征,考察在不同的pH值和温度的条件下α-葡萄糖苷酶的酶活力,测定两者对α-葡萄糖苷酶的抑制作用,并采用双倒数作图法研究其动力学特性。结果:合成的木犀草素-Cr(Ⅲ)配合物的分子组成为:C15H9O6Cr(COOCH3)2(H2O)2·2H2O;酶的最适作用pH值为5.0,温度为55 e;木犀草素及其配合物对α-葡萄糖苷酶抑制作用的IC50值分别为1.496 mmol/L、0.2320 mmol/L,抑制类型为竞争型α-葡萄糖苷酶抑制剂。结论:木犀草素与Cr3+结合后,增强了对α-葡萄糖苷酶的抑制作用,为木犀草素-Cr(Ⅲ)的进一步研究打下了良好的基础。
OBJECTIVE: To synthesize luteolin-Cr (Ⅲ) and study the inhibitory effect of luteolin and luteolin-Cr (Ⅲ) on α-glucosidase and determine the type of inhibition. Methods: Luteolin-Cr (III) complex was synthesized by stirring with luteolin and chromium acetate in anhydrous ethanol. The structure was characterized by IR, UV, TGDTA and elemental analysis. The effects of different pH and temperature The enzyme activity of α-glucosidase was measured. The inhibitory effect of α-glucosidase and β-glucosidase was determined. The kinetic properties of α- Results: The molecular composition of the synthesized luteolin-Cr (Ⅲ) complex was: C15H9O6Cr (COOCH3) 2 (H2O) 2 · 2H2O. The optimum pH of the enzyme was 5.0 and the temperature was 55e. The IC50 values of the complexes for α-glucosidase inhibition were 1.496 mmol / L and 0.2320 mmol / L, respectively, and the inhibitory type was competitive α-glucosidase inhibitor. Conclusion: The combination of luteolin and Cr3 + enhances the inhibitory effect on α-glucosidase, laying a good foundation for the further study of luteolin-Cr (Ⅲ).