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本文用Ｔｒｉｔｏ－溶菌酶法制备质粒ＤＮＡ，氯化铯梯度离心，经ＨｉｎｄⅢ和ＥｃｏＲＩ双酶切后，电泳分离６８０ｂｐＤＮＡ片段，得到Ｔ细胞受体β链（ＴＣＲβ）Ｃ区探针。抽提２８例Ｇｒａｖｅｓ病（ＧＤ）患者和４２例正常人外周血ＤＮＡ，经ＢｇｌⅡ酶切后，Ｓｏｕｔｈｅｒｎ转移至尼龙膜上，与按随机法标记的探针杂交。结果发现有三种基因表现型即１０／１０Ｋｂ，１０／９．２Ｋｂ，９．２／９．２Ｋｂ，其中ＧＤ患者１０／９．２Ｋｂ杂合子出现频率明显高于正常对照组（分别为０．７１，０．３８，Ｐ＜０．０１），而ＧＤ患者１０／１０Ｋｂ纯合子出现频率则明显低于正常对照组（分别为０．０７，０．３１，Ｐ＜０．０１）。提示ＴＣＲβ限制性内切酶片段多态性（ＲＦＬＰ）ＢｇｌⅡ与ＧＤ患者的易感性有关联
In this paper, plasmid DNA was prepared by Trito-lysozyme method and cesium chloride gradient centrifugation. After digested with HindIII and EcoRI, the 680 bp DNA fragment was separated by electrophoresis to obtain probe C of T cell receptor beta chain (TCR beta). Peripheral blood DNA was extracted from 28 patients with Graves’ disease (GD) and 42 healthy controls. After digested with BglII, the cells were transferred to nylon membrane and hybridized with randomly labeled probes. The results showed that there are three kinds of gene expression that 10 / 10Kb, 10 / 9.2Kb, 9.2 / 9.2Kb, GD patients with 10 / 9.2Kb heterozygosity frequency was significantly higher than the normal control group (0.71 , 0.38, P <0.01). However, the frequencies of 10 / 10Kb homozygotes in GD patients were significantly lower than those in normal controls (0.07,0.31, P <0.01, respectively). Tip TCRβ restriction fragment polymorphism (RFLP) Bgl Ⅱ associated with the susceptibility of GD patients