论文部分内容阅读
目的通过对大鼠实验前给予左旋-硝基精氨酸甲脂(L-NAME,NO前体左旋精氨酸类似物)后,观察急性高眼压后大鼠视网膜电图(ERG)b波幅的变化,iNOSmRNA的表达情况。探讨L-NAME和i-NOS在高眼压视网膜损伤中的作用。方法Wister大鼠48只随机分为6组,分别为高眼压30min、90min组、高眼压后12h组、注射L-NAME+高眼压30min、90min组和注射L-NAME+高眼压后12h组。前房加压灌注成高眼压模型。Alnplaldamk15型视电生理仪检测FERG-b波波幅变化。RT-PCR法检测iNOSmRNA的表达。结果L-NAME组的ERG-b波波幅与对照组相比前者明显恢复(P<0.01)。高眼压30min,90min,12hiNOSmRNA的表达逐渐增强,但L-NAME组中iNOSmRNA的表达明显低于对应的高眼压组(P<0.01)。结论L-NAME做为一氧化氮合酶的抑制剂,通过抑制iNOS合成具有细胞毒性的NO,有助于缺血后的视网膜功能的恢复,对视网膜起到保护作用。
OBJECTIVE: To observe the changes of ERG (b) amplitude after acute ocular hypertension in rats by administering L-NAME (L-arginine analogues of NO precursor) Changes in iNOS mRNA expression. To investigate the role of L-NAME and i-NOS in retinal damage after ocular hypertension. Methods Forty-eight Wister rats were randomly divided into 6 groups: high pressure intraocular pressure (IOP) for 30min and 90min, intraocular pressure (Ih) and intraocular pressure group. Anterior chamber pressure perfusion into high intraocular pressure model. Alnplaldamk15 type electrophysiology instrument to detect changes in FERG-b wave amplitude. The expression of iNOS mRNA was detected by RT-PCR. Results The amplitude of ERG-b in L-NAME group was significantly higher than that in control group (P <0.01). The expression of 12hiNOS mRNA increased gradually after 30min, 90min, but the expression of iNOS mRNA in L-NAME group was significantly lower than that in corresponding ocular hypertension group (P <0.01). Conclusion L-NAME, as a nitric oxide synthase inhibitor, inhibits the synthesis of cytotoxic NO by iNOS and contributes to the recovery of ischemic retinal function and plays a protective role on the retina.