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目的 研究人类免疫缺陷病毒 1型 (HIV 1)感染无进展与进展者HIVDNA存在状态的特点。方法 选用位于HIV 1DNA链两端的LTR(U5 )、LTR(R)等其他部位的不同引物 ,应用国际上新颖的长片段聚合酶链反应 (LD PCR)及逆转录PCR(RT PCR) ,普通PCR(Taq PCR)、免疫转印、分子杂交等技术 ,对HIV 1感染者血清HIVRNA、外周血单个核细胞 (PBMC)HIVDNA进行检测。结果 9.1kb为全长HIVDNA基因片段 ,12例进展者全部存在全长HIV基因片段 ,其中 ,9例存在不完整缺损的HIVDNA ;在 18例无进展者中 ,5例既存在完整又存在不完整的HIVDNA ,其余13例只存在不完整、缺损的HIVDNA。结论 完整和缺损HIVDNA与临床表现类型有密切关系
Objective To investigate the status of the presence of HIV-1 infection in HIV-1 non-HIV-infected and progression-free human immunodeficiency virus type 1 (HIV-1) patients. Methods The different primers of LTR (U5), LTR (R) and other loci located at both ends of HIV 1 DNA chain were used. The novel PCRs and reverse transcription PCR (RT - PCR) (HIV), HIV-1 and peripheral blood mononuclear cells (PBMC) were detected by Taq PCR, immunoblotting and molecular hybridization. The results of 9.1kb for the full-length HIVDNA gene fragments, 12 cases of full-length HIV gene fragments all exist, of which 9 cases of incomplete deletion of HIVDNA; 18 cases of non-progrester, there are 5 cases both complete and incomplete Of the HIVDNA, the remaining 13 cases there is only incomplete, defective HIVDNA. Conclusion The complete and defect HIVDNA is closely related to the clinical manifestations