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The effects of La3+ and Gd3+ on the proliferation, differentiation and adipogenic transdifferentiation of rat calvarial osteoblast-like cells (ROB cells) were evaluated by MTT method, measuring the activity of alkaline phosphatase (ALP) and Oil red O measurement. Both of La3+ and Gd3+ inhibited the proliferation of ROB cells at all concentrations (1×10-5, 1×10-6, 1×10-7, 1×10-8, 1×10-9 mol·L-1). La3+ at concentration of 1×10-5mol·L-1 significantly increased the alkaline phosphatase activity of ROB cells up to 3 folds (P<0.01). However, the effects reversed to inhibit at other concentrations. Gd played a negative role in the alkaline phosphatase activity. La3+ inhibited the adipogenic transdifferentiation of ROB cells at all concentrations in a dose-dependent way. However, Gd promoted the adipogenic transdifferentiation of ROB cells at 1×10 and 1×10 mol·L . These findings suggested that the effects of rare earth elements on the proliferation, differentiation and adipogenic transdifferentiation of ROB cells were dependent on their concentrations and species.
The effects of La3 + and Gd3 + on the proliferation, differentiation and adipogenic transdifferentiation of rat calvarial osteoblast-like cells (ROB cells) were evaluated by MTT method, measuring the activity of alkaline phosphatase (ALP) and Oil red O measurement. Both of La3 + and Gd3 + inhibited the proliferation of ROB cells at all concentrations (1 × 10-5, 1 × 10-6, 1 × 10-7, 1 × 10-8, 1 × 10-9 mol·L-1) of 1 × 10-5mol·L-1 significantly increased the alkaline phosphatase activity of ROB cells up to 3 folds (P <0.01). However, the applied values of Gd played a negative role in the alkaline phosphatase activity . La3 + inhibited the adipogenic transdifferentiation of ROB cells at all concentrations in a dose-dependent way. However, Gd promoted the adipogenic transdifferentiation of ROB cells at 1 × 10 and 1 × 10 mol·L. These findings suggest that the effects of rare earth elements on the proliferation, differentiation and adipogenic tr ansdifferentiation of ROB cells were dependent on their concentrations and species.