论文部分内容阅读
目的:研究丙戊酸(VPA)浓度和干预时间对神经干细胞(NSCs)体外分化的影响。方法:以不同浓度的VPA(0.1、0.3、0.5、0.75和1.0mmol/L)处理原代培养的神经干细胞,以NB培养基组做对照,分别于神经干细胞分化后3天、7天、10天和14天用免疫荧光双标鉴定并计数微管蛋白-Ⅲ(β-tubllin III)和胶质原纤维酸性蛋白(GFAP)阳性细胞的比例,并作统计学分析。结果:同一时相点组间比较,3天时各组中神经元分化比例无显著差异;7天时不同浓度VPA组与对照组分化神经元比例开始呈现差异;10天时这种差异继续增大,0.75 mmol/L VPA组中神经元比例为82.15±0.93%;14天时保持这种差异;但各时相点1.0 mmol/L VPA组与0.75 mmol/L VPA组神经元分化的比例无显著差异。不同时相点组内比较发现,3-10天内随着时间的延长,各组神经元分化的比例显著增加,但14天时神经元分化的比例较10天无显著变化。结论:0.75mmol/L VPA在10天时促神经干细胞向神经元分化的作用最佳。
Objective: To investigate the effect of VPA concentration and intervention time on the differentiation of neural stem cells (NSCs) in vitro. Methods: Primary cultured neural stem cells were treated with different concentrations of VPA (0.1,0.3,0.5,0.75 and 1.0mmol / L), respectively. NB medium group was used as control. The neural stem cells were cultured for 3 days, 7 days, 10 days Day and fourteen days by immunofluorescence double labeling identified and counted tubulin-III (β-tubllin III) and glial fibrillary acidic protein (GFAP) positive cells ratio, and for statistical analysis. Results: At the same time points, there was no significant difference in the proportion of neurons in each group at 3 days. Differences in the proportion of differentiated neurons between VPA group and control group began to show differences at 7 days. At 10 days, the difference continued to increase. 0.75 The proportion of neurons in the group of mmol / L VPA was 82.15 ± 0.93%, while the difference was maintained on the 14th day. There was no significant difference in the proportion of neurons between the group of 1.0 mmol / L VPA and the group of 0.75 mmol / L VPA at each time point. Compared with different time points, the proportion of neuron differentiation in each group increased significantly with time prolonging in 3-10 days, but the proportion of neuron differentiation did not change significantly in 14 days. Conclusion: 0.75mmol / L VPA can promote the differentiation of neural stem cells into neurons at 10 days.