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目的:观察降钙素基因相关肽(calcitonin-gene-related peptide,CGRP)样阳性终末在中央杏仁核(cen-tral nucleus of amygdala,CeA)内形成的突触的超微结构。方法:应用免疫荧光组织化学和包埋前免疫电镜等方法,观察CGRP样阳性终末在CeA内所形成的突触分布形式及结构特点。结果:CGRP样阳性终末在中央杏仁核内可以与细胞体、树突干和树突棘等结构形成突触;轴-体突触几乎全为对称性突触,而轴-树突触和轴-棘突触则多为非对称性突触。在所有的非对称性突触里,轴-树突触占84.9%,而轴-棘突触占15.1%。CGRP样阳性轴-树的突触后致密带的平均长度为(790.77±313.55)nm,而轴-棘突触的突触后致密带的平均长度为(723.34±357.20)nm,两者之间没有显著性差异。结论:CGPR样阳性的兴奋性突触尤其是轴-树突触在伤害性信息传递以及痛相关情绪的产生中发挥了重要作用。
OBJECTIVE: To observe the ultrastructure of synapses formed by calcitonin-gene-related peptide (CGRP) -positive terminal in cen-tral nucleus of amygdala (CeA). Methods: Immunofluorescence histochemistry and immunoelectron microscopy before embedding were used to observe the synaptic distributions and structural features of CGRP-like terminals in CeA. RESULTS: CGRP-like positive terminals formed synapses in the central amygdala with cellular bodies, dendrites, and dendritic spines. Axonal-synapses were almost exclusively symmetrical and axonal-synaptic and Axis - spinous processes are mostly asymmetric synapses. Axis-dendrites accounted for 84.9% of all asymmetric synapses, while axis-spinous processes accounted for 15.1%. The average length of the postsynaptic dense band of CGRP-like axis-axis was (790.77 ± 313.55) nm, while the average length of the synaptic cleft in the axis-spinous process was (723.34 ± 357.20) nm No significant difference. CONCLUSIONS: CGPR-like excitatory synapses, particularly axonal-dendritic synapses, play an important role in the transmission of nociceptive information and in the development of pain-related emotions.