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目的 探讨超声微泡造影剂对心肌组织的生物学效应及其介导VEGF基因转染大鼠心肌的有效性。方法 18只健康雄性Wistar大鼠 ,取 3只采用超声波在鼠胸壁破坏微泡造影剂 ,观察对心肌组织显微结构的影响。将另 15只急性心肌梗死 3天后的雄性Wistar大鼠分为 3组 ,每组 5只。第一组采用超声破坏微泡造影剂的方式 ,将pcD2 VEGF12 1基因转染大鼠心肌至造影剂不再显影 (约 6min) ;第二组尾静脉输入同等剂量携pcD2 VEGF12 1基因的造影剂 ;第三组为对照。 2周后 ,取缺血心肌组织行VEGF免疫组织化学染色 ,观察心肌组织血管内皮生长因子 (VEGF)蛋白表达情况。结果 超声波破坏微泡造影剂能使心肌组织充血 ,产生大量空泡 ,并有部分心肌细胞坏死。采用超声微泡造影剂介导的VEGF基因转染 ,能明显增强大鼠心肌组织VEGF蛋白的表达。结论 超声微泡造影剂能明显增强对组织的空化效应 ,其介导的VEGF基因治疗是一种无创、新型、高效的基因转移方法。
Objective To investigate the biological effect of ultrasound microbubble contrast agent on myocardial tissue and the effect of VEGF gene transfection on myocardium in rats. Methods Eighteen healthy male Wistar rats were randomly divided into three groups. Ultrasound was used to destroy the microbubble contrast agent in the mouse chest wall to observe the effect on myocardial microstructure. The other 15 Wistar rats, three days after acute myocardial infarction, were divided into three groups of five. In the first group, the microbubble contrast agent was subdivided by ultrasound, and then the pcD2 VEGF12 1 gene was transfected into the myocardium of rats to no longer develop the contrast medium (about 6 minutes). The second group received the same dose of contrast agent carrying pcD2 VEGF12 1 gene ; The third group as a control. Two weeks later, the ischemic myocardium was taken for VEGF immunohistochemical staining to observe the expression of vascular endothelial growth factor (VEGF) protein in myocardium. Results ultrasound damage microbubble contrast agents can make myocardial tissue congestion, resulting in a large number of vacuoles, and some myocardial cell necrosis. The VEGF gene transfection mediated by ultrasound microbubble contrast agent can obviously enhance the expression of VEGF protein in rat myocardium. Conclusion Ultrasound microbubble contrast agent can significantly enhance the cavitation effect on the tissue. VEGF-mediated gene therapy is a non-invasive, novel and efficient gene transfer method.