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[Objectives] To establish fingerprint chromatogram of Tetracera asiatica and provide basis for controlling and assessing the quality of Tetracera asiatica.[Methods] The high-performance liquid chromatography( HPLC) method was used. Phenomenex C18( 5 μm,4. 6 ×250 mm),acetonitrile-0. 1% phosphoric acid water,gradient elution,flow rate 1 m L/min,detection wavelength 214 nm,and column temperature 25 ℃. The fingerprint chromatogram of 10 batches of samples of Tetracera asiatica was determined for similarity comparison. [Results] It established HPLC fingerprint chromatogram of Tetracera asiatica produced in Guangxi,determined a total of 13 common fingerprint peaks. The similarity of 10 batches of Tetracera asiatica was > 0. 9.[Conclusions] The method is rapid and accurate and can provide references for assessing the quality of Tetracera asiatica.
[Methods] The high-performance liquid chromatography (HPLC) method was used. Phenomenex C18 (5 μm, 4.6 × 250 mm), acetonitrile-0.1% phosphoric acid water, gradient elution, flow rate 1 m L / min, detection wavelength 214 nm, and column temperature 25 ° C. The fingerprint chromatogram of 10 batches of samples of Tetracea asiatica was determined for similarity comparison. [Results] It established HPLC fingerprint chromatogram of Tetracera asiatica produced in Guangxi, determined a total of 13 common fingerprint peaks. The similarity of 10 batches of Tetracera asiatica was> 0. 9. [Conclusions] The method is rapid and accurate and can provide references for assessing the quality of Tetracera asiatica.