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目的:血清反应因子在与心血管相关的疾病基因调控方面的作用越来越重要。血清反应因子识别的结合位点CArG元件因其重要的基因调控作用近年来随之备受关。本研究目的是揭示血清因子结合位点的位置分布与功能的关系及CArG元件内部各个位点的保守性。方法:本研究应用生物信息学方法结合遗传学方法对小鼠中CArG元件的位置分布、位点替换率及GO分类进行深入研究。结果:结果表明,71%的功能CArG元件分布在转录起始位点上游,且距离转录起始位点越近,CArG元件的数量越多。保守性分析发掘出元件内部的替换冷点、热点及替换规律。GO分类结果显示,CArG依赖性基因多为信号转导和细胞骨架蛋白。结论:上述研究结果将为准确预测候选CArG元件提供重要理论基础,同时也将为更为深入阐述SRF的调控模式奠定基础。
OBJECTIVE: The role of serum response factors in gene regulation of cardiovascular-related diseases is of increasing importance. Serum response factor recognition site CArG elements because of its important role in gene regulation has been followed in recent years. The purpose of this study was to reveal the relationship between the location and function of serum factor binding sites and the conservation of each site within the CArG element. Methods: In this study, bioinformatics methods combined with genetic methods of CArG components in mice location distribution, site replacement rate and GO classification in-depth study. Results: The results showed that 71% of the functional CArG elements distributed upstream of the transcription start site, and the closer to the transcription start site, the more the number of CArG elements. Conservative analysis to find out the components within the replacement of cold spots, hot spots and replacement rules. GO classification results showed that, CArG-dependent genes are mostly signal transduction and cytoskeletal proteins. Conclusion: The results of the above studies will provide important theoretical basis for accurate prediction of candidate CArG components, and will lay a foundation for further elaborating the regulatory mode of SRF.