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以‘晋谷45’和‘晋谷42’2个品种作为试验材料,采用聚乙二醇(PEG-6000)模拟干旱胁迫处理谷子幼苗,检测不同处理时间下丙二醛(MDA)的含量,评价其抗旱性。此外,在2个品种中克隆获得SiZFP182基因的cDNA全长,分析比较它们之间的序列差异;通过定量RT-PCR的方法分析SiZFP182基因在受到干旱胁迫的谷子幼苗中的表达特性。结果表明,‘晋谷45’的抗旱性强于‘晋谷42’,2个品种中SiZFP182基因的序列存在5处单碱基差异,使得编码的氨基酸序列不同。此外,PEG胁迫处理后24h内,SiZFP182基因在2个品种中的表达水平均表现出先升高后降低的趋势,处理后6~12h,该基因在‘晋谷45’中表达水平高于‘晋谷42’。初步认为‘晋谷45’的抗旱特性与‘晋谷42’对干旱敏感的特性,可能与干旱胁迫下处于幼苗期的2个品种中SiZFP182基因序列差异及表达差异相关。
The seedlings of millet were treated with polyethylene glycol (PEG-6000) to simulate drought stress, and the content of malondialdehyde (MDA) under different treatment time was measured with ’Jingu 45’ and ’Jin’gu 42’2 cultivars. Evaluate its drought resistance. In addition, the full-length cDNA of SiZFP182 was cloned from two cultivars, and their sequence differences were analyzed and compared. The expression of SiZFP182 in millet seedlings under drought stress was analyzed by quantitative RT-PCR. The results showed that ’Jinyu 45’ had stronger drought resistance than ’Jinyu 42’. There were 5 single-base differences in the sequence of SiZFP182 in the two cultivars, which resulted in different amino acid sequences. In addition, the expression level of SiZFP182 gene in two cultivars showed the trend of first increasing and then decreasing after 24h of PEG stress treatment. The expression level of SiZFP182 gene was higher in Jingu 45 after 6-12h treatment Valley 42 ’. It is preliminarily believed that the drought resistance characteristics of ’Jingu 45’ and drought-sensitive characteristics of ’Jingu 42’ may be related to the differences in sequence and expression of SiZFP182 in two cultivars under drought stress.