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本研究以两种灭活痘病毒属病毒(vaccinia virus,7.0×10~6 PFU和cowpox virus,9.2×10~7 PFU)为免疫原,分别考察不同剂量下(抗原浓度相差约100倍)实验鸡的免疫效果和相应卵黄抗体效价(免疫荧光法和抗体中和效价)。我们的研究结果,结合文献研究显示,抗原剂量对于特异性卵黄抗体的产生和表达水平具有重要影响。提高免疫原的浓度通常有助于提高抗体效价。但由于不同抗原的免疫原性差异,也有可能导致免疫抑制。针对不同免疫原似乎存在最佳免疫剂量范围。
In this study, two kinds of vaccinia virus (7.0 × 10 ~ 6 PFU and cowpox virus, 9.2 × 10 ~ 7 PFU) were used as immunogens to study the effects of different doses (the difference of antigen concentration was about 100 times) Immunogenicity of chicken and corresponding yolk antibody titers (immunofluorescence and antibody neutralization titers). Our results, combined with literature studies, have shown that antigen dosage has a significant impact on the production and expression of specific yolk antibodies. Increasing the concentration of the immunogen generally helps to increase the antibody titer. However, due to differences in the immunogenicity of different antigens, it is also possible to cause immunosuppression. There seems to be the best range of immunization doses for different immunogens.