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目的 探索外源性血小板源伤口愈合因子 (platelet -derivedwoundhealingfactors,PDWHF)对糖尿病因难愈性伤口愈合的改善作用及其相关的分子机制。方法 四氧嘧啶诱导SD大鼠血糖值 >1.8mg/L后 ,采用大鼠背部成对切口伤模型 ,一侧伤口局部应用PDWHF( 10 0 μg/切口 ) ,手术当日及术后连续 6日每日 1次 ,另一侧作为自身对照。体外培养肉芽组织成纤维细胞 ,生长融合后 ,加入 10 %PDWHF ( 5g/L) ,观测前胶原Ⅰ (α1)mRNA水平量。结果 伤后 7,10 ,14天治疗组与自身对照非治疗组伤口抗张力值分别为 ( 10 3± 13 ) ,( 2 4 9± 4 9) ,( 5 4 6± 3 7)g/cm和 ( 67± 12 ) ,( 113± 2 8) ,( 2 77± 3 0 )g/cm ,两组间差异显著 (P <0 .0 1)。体外实验发现 :生长融合的伤口成纤维细胞与PDWHF孵育 4 ,8,12小时后 ,PDWHF作用组前胶原Ⅰ (α1)mRNA分别较对照组高 0 .9,3 .7,2 .2倍。结论 PDWHF促进糖尿病大鼠伤口愈合与其直接刺激伤口成纤维细胞前胶原Ⅰ (α1)基因表达相关。
Objective To explore the effect of exogenous platelet-derived wound healing factor (PDWHF) on the healing of diabetic refractory wounds and its related molecular mechanisms. Methods Alloxan-induced SD rat blood glucose> 1.8mg / L, the rat’s back paired incision injury model, PDWHF (100 μg / incision) was applied on one side of the wound, on the 6th day Day 1, the other side as their own control. Granulation tissue fibroblasts were cultured in vitro. After the growth and fusion, 10% PDWHF (5g / L) was added to observe the amount of procollagen Ⅰ (α1) mRNA. Results The anti-tonic values of the wounds in the untreated group and the untreated group were (10 3 ± 13), (24 9 ± 4 9), (54 6 ± 37) g / cm And (67 ± 12), (113 ± 2 8) and (2 77 ± 30) g / cm, respectively. There was significant difference between the two groups (P <0.01). In vitro experiments showed that PDWHF-treated group exhibited a significant increase of procollagen Ⅰ (α1) mRNA by 0.9, 3.7, 2.times.2 times higher than that of the control group at 4, 8, and 12 hours after the fusion of wound fibroblasts were incubated with PDWHF. Conclusion PDWHF can promote wound healing in diabetic rats and directly stimulate the expression of procollagen Ⅰ (α1) gene in wound fibroblasts.