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本研究从30个母系全同胞家系中各选取30尾牙鲆,28℃高温养殖40d,运用最小二乘法构建各牙鲆家系在高温下生长40d后的体质量(Y)与初始的体质量(X)的回归方程,结果为:Y=2.433X+5.539,(R=0.899),其中斜率的95%置信区间为1.973~2.892,截距的95%置信区间为0.909~10.169,并最终得到高温情况下各个家系增重情况的遗传力的估算值在0.15~0.38之间,属于中等遗传力。以牙鲆家系为材料进行了急性温度致死和慢性温度致死实验,实验结果表明:实验温度的选取在耐高温选育过程中至关重要,急性温度致死实验中(32℃)各家系均在58h左右死亡;而在慢性温度致死实验(31℃)中,各家系表现出了良好的耐热选择效应,死亡时间呈现了一定的连续性,各家系间耐热性差异极显著(P<0.01)。在31℃下,根据其热致死时间对其耐热性选择,最终选择2、11、19和36号家系作为耐热家系,为下一步耐热家系选育提供了基础群体。
In this study, 30 Paralichthys olivaceus were selected from 30 maternal full-sib families and cultured at 28 ℃ for 40 days. The body mass (Y) and initial body mass of each Paralichthys olivaceus family grown 40 days at high temperature X), the result is: Y = 2.433X + 5.539, (R = 0.899), where the 95% confidence interval of the slope is 1.973 ~ 2.892 and the 95% confidence interval of the intercept is 0.909 ~ 10.169, finally the high temperature The estimated heritability of each family weight gain in the case of 0.15 ~ 0.38, belonging to the middle heritability. The experimental results showed that the selection of experimental temperature is very important in the process of high temperature selection and breeding. All the families in the acute temperature lethality experiment (32 ℃) 58h, respectively. However, in the experiment of lethal temperature death (31 ℃), all the families showed good heat-resistant selection effect, the death time showed a certain continuity, and the difference of heat resistance among the families was extremely significant (P <0.01). At 31 ℃, according to its heat-tolerant time to heat-resistant selection, the final selection of 2,11,19 and 36 families as heat-resistant families, for the next heat-resistant pedigree provide a basic group.