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目的建立一种新型的靶向高效诱发肿瘤血管血栓栓塞体系磁流体-神经纤维网蛋白1抗体A6-链霉亲和素:生物素-短截型组织因子(MF-A6-SA:B-tTF)。方法化学交联技术制备神经纤维网蛋白1抗体A6-链霉亲和素、磁流体-神经纤维网蛋白1抗体A6-链霉亲和素和生物素-短截型组织因子交联物,凝血因子X活化实验鉴定复合体系活化凝血因子X的活力,荧光显微镜技术和普鲁士蓝染色法同时观察施加外界磁场后复合体系的靶向作用,凝血实验直接观察复合体系引入链霉亲和素:生物素的生物放大效应,体内生物分布实验观察复合体系的安全性。结果成功制备磁流体-神经纤维网蛋白1抗体A6-链霉亲和素及生物素-短截型组织因子,磁流体-神经纤维网蛋白1抗体A6-链霉亲和素:生物素-短截型组织因子体系保留有高效激活凝血因子X的活性,与靶点的结合具有靶向性及高效富集性,体内实验证实能安全有效诱发肿瘤血管栓塞。结论成功制备的具有靶向诱发肿瘤血管血栓栓塞体系磁流体-神经纤维网蛋白1抗体A6-链霉亲和素:生物素-短截型组织因子为进一步探索肿瘤血管的靶向治疗奠定基础。
OBJECTIVE: To establish a novel target-directed and efficient tumor-associated vascular thromboembolism system, magnetofluorescence-neurofibrin-1 antibody A6-streptavidin: biotin-stranded tissue factor ). Methods The chemical cross-linking technique was used to prepare the neurofibrin-1 antibody A6-streptavidin, magnetic fluid-neurofibrin-1 antibody A6-streptavidin and biotin-stranded tissue factor cross- Factor X activation assay to identify the activity of the composite activated coagulation factor X, fluorescence microscopy and Prussian blue staining simultaneously observed the application of the external magnetic field after the composite system of the role of the coagulation test direct observation of the composite system into the streptavidin: biotin The biological amplification effect, in vivo biodistribution experiment to observe the safety of the composite system. Results The magnetic fluid-neurofibrin-1 antibody A6-streptavidin and biotin-stranded tissue factor were successfully prepared. Magnetic fluid-neurofibrin-1 antibody A6-streptavidin: biotin-short Tissue factor system retains the high activity of activated coagulation factor X activity, binding with the target with high efficiency and enrichment, in vivo experiments confirmed that safe and effective induction of tumor vascular embolism. Conclusion A6-streptavidin-biotin-short stranded tissue factor (MHDF-1) with target-induced tumor vascular thromboembolism was successfully prepared for further exploration of tumor vascular targeting therapy.