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目的检测精子相关抗原9(SPAG9)在卵巢浆液性上皮肿瘤中的表达,初步探讨其在卵巢浆液性上皮肿瘤术前诊断中的意义。方法应用免疫组织化学SP法,检测良性组24例、交界性组27例、恶性组32例中SPAG9的表达,采用酶标记免疫吸附测定法(ELISA)检测卵巢浆液性上皮肿瘤患者血清中SPAG9的表达水平,与对应的血清CA125表达水平进行相关性分析,并用受试者工作特征(ROC)曲线评价两指标的诊断价值。结果①SPAG9在卵巢上皮性恶性肿瘤组织中的表达高于交界性组及良性组,并与恶性肿瘤的细胞分化程度相关(P<0.05);②卵巢浆液性上皮性肿瘤患者血清中SPAG9有表达,恶性组高于良性组,其血清学水平与CA125呈正相关(P<0.01),ROC曲线分析提示SPAG9对卵巢上皮性恶性肿瘤有较高的诊断意义,但并不优于CA125。结论SPAG9的表达与卵巢上皮性肿瘤的恶性程度相关,在卵巢上皮性恶性肿瘤中表达较高,可作为卵巢上皮性肿瘤术前诊断和术后治疗监测新的生物学指标。
Objective To detect the expression of sperm-associated antigen 9 (SPAG9) in ovarian serous epithelial tumors and to explore its significance in the preoperative diagnosis of ovarian serous epithelial tumors. Methods Immunohistochemical SP method was used to detect the expression of SPAG9 in 24 cases of benign group, 27 cases of borderline group and 32 cases of malignant group. The level of SPAG9 in serum of ovarian serous epithelial tumor was detected by enzyme-linked immunosorbent assay (ELISA) The correlation between serum CA125 levels and the corresponding serum levels of CA125 was analyzed. The ROC curve was used to evaluate the diagnostic value of the two indicators. Results ① The expression of SPAG9 in epithelial ovarian cancer was higher than that in borderline and benign groups, and was correlated with the degree of cell differentiation (P <0.05). ②The expression of SPAG9 in ovarian serous epithelial tumors was significantly higher than that in benign ovarian tumors Serum level of malignant group was significantly higher than that of benign group (P <0.01). The ROC curve analysis indicated that SPAG9 was of high diagnostic value for ovarian epithelial malignant tumor, but not superior to CA125. Conclusion The expression of SPAG9 is correlated with the malignant degree of ovarian epithelial tumor and high expression in epithelial ovarian cancer. It may be used as a new biological indicator for the preoperative diagnosis and postoperative treatment of ovarian epithelial tumor.