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目的研究镧对学习记忆的影响,并从大脑皮质即刻早期基因表达的角度探讨镧影响学习记忆的机制。方法40只成年雌性Wistar大鼠随机分成对照组和低、中、高剂量染镧组,染镧组仔鼠在出生至断乳后1个月期间染镧,然后进行神经行为学测试(水迷宫和穿梭箱测试),测定大脑皮质中c-fosmRNA和c-Fos蛋白以及尼氏体表达情况。结果神经行为学测试结果显示染镧组仔鼠的学习记忆能力明显低于对照水平,呈现一定的量-效关系。低剂量染镧组仔鼠大脑皮质c-fosmRNA和c-Fos蛋白表达水平明显低于对照水平,中剂量染镧组仔鼠海马c-fosmRNA表达水平明显低于对照水平,c-Fos蛋白表达水平明显低于对照和低剂量染镧组,高剂量染镧组仔鼠大脑皮质c-fosmRNA和c-Fos蛋白表达水平均明显低于对照和低剂量染镧组。另外,低剂量染镧组大脑皮质神经细胞中尼氏体表达水平明显低于对照水平,中、高剂量染镧组大脑皮质神经细胞中尼氏体表达水平进一步减少。结论染镧造成学习记忆能力下降,这可能与染镧引起大脑皮质即刻早期基因的基因和蛋白表达水平下降、神经细胞功能削弱有一定关系。
Objective To study the effect of lanthanum on learning and memory and to explore the mechanism of lanthanum-influenced learning and memory from the perspective of early gene expression in the cerebral cortex. Methods Forty adult female Wistar rats were randomly divided into control group and low, medium and high dose groups of lanthanum. The lanthanum group was lased with lanthanum for one month from birth to weaning, and then neurobehavioral test (water maze And shuttle box test) to determine c-fos mRNA and c-Fos protein and Nissl expression in the cerebral cortex. Results The neurobehavioral test results showed that the learning and memory abilities of the pups of the lanthanum-doped group were significantly lower than those of the control group, showing a dose-effect relationship. The expression of c-fos mRNA and c-Fos protein in cerebral cortex of low-dose lanthanum group was significantly lower than that of the control group. The expression of c-fos mRNA in hippocampus of medium dose lanthanum group was significantly lower than that of the control group Significantly lower than the control group and the low-dose lanthanum group. The expression of c-fos mRNA and c-Fos protein in cerebral cortex of the high-dose lanthanum-loaded group were significantly lower than those of the control group and the low-dose lanthanum group. In addition, the expression of Nissl in cerebral cortical neurons of low-dose lanthanum-treated group was significantly lower than that of control group, while the expression of Nissl in cerebral cortex neurons of medium and high-dose lanthanum group was further reduced. Conclusion Dye lanthanum can reduce the ability of learning and memory, which may be related to the decrease of gene and protein expression of early gene in the cerebral cortex, and the impaired function of nerve cells.