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目的:考察盾叶薯蓣同源四倍体的遗传稳定性。方法:利用显微方法进行根尖染色体鉴定;于生长稳定期观测田间苗的农艺性状;测定蛋白质含量并进行蛋白质聚丙烯酰胺凝胶电泳;测定和比较薯蓣皂苷元含量。结果:盾叶薯蓣四倍体株系的染色体数目仍为40,农艺性状保持了器官巨型性的多倍体特征,各株系蛋白质电泳谱带基本相似,薯蓣皂苷元及蛋白质含量均高于二倍体对照株系。结论:本实验证实人工诱导的盾叶薯蓣同源四倍体具有较好的遗传稳定性,为后续的多倍体优良品种选育工作奠定了基础。
Objective: To investigate the genetic stability of autotetraploid Dioscorea zingiberensis. Methods: The apical chromosomes were identified by microscopy. The agronomic traits of field seedlings were observed during the growth phase. The protein content was measured and subjected to polyacrylamide gel electrophoresis. The content of diosgenin was determined and compared. Results: The number of tetraploid tetraploid strains of Dioscorea zingiberensis was 40, and the agronomic traits retained the giant polyploidy of organs. The electrophoretic bands of the proteins in each strain were similar, and the contents of diosgenin and protein were higher than those of Di Ploidy control strains. Conclusion: This experiment confirmed that artificial induction of autotetraploid Dioscorea zingiberensis CH Wright has a good genetic stability, which laid the foundation for subsequent polyploid breeding.