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目的探讨单核细胞趋化因子-1(MCP-1)受体CCR2基因190A/G、烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶p22phox亚基基因C242T多态性与吸烟的交互作用与非酒精性脂肪性肝病(NAFLD)的关系。方法采用病例-对照研究的方法,以600例NAFLD患者及600例健康对照者的外周血白细胞为样本,利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析了MCP-1受体CCR2基因190A/G和NADPH氧化酶p22phox亚基基因C242T多态性。结果 190A/G(GG)基因型和C242T(TT)基因型频率分布在病例组分别为50.17%、50.00%,在对照组分别为23.83%、24.17%,二者经χ2检验差异有统计学意义(χ2=88.8462,P=0.0031;χ2=85.8100,P=0.0039)。190A/G(GG)基因型者患NAFLD的风险显著增加(OR=3.2171,95%CI 1.9351~5.2184)。C242T(TT)基因型者患NAFLD的风险也显著增加(OR=3.1379,95%CI 1.7973~5.2362)。基因突变的协同分析发现190A/G(GG)/C242T(TT)基因型者在NAFLD组和对照组中的分布频率分别为39.67%和13.00%,二者经χ2检验差异有统计学意义(χ2=118.3021,P=0.0017)。190A/G(GG)/C242T(TT)基因型者患NAFLD的风险显著增加(OR=5.0211,95%CI 3.1853~7.7926)。病例组的吸烟率显著高于对照组(χ2=92.2234,P=0.0025),吸烟者患NAFLD的风险显著增加(OR=3.3032,95%CI 1.9147~5.7413),吸烟与190A/G(GG)/C242T(TT)基因型均有交互作用(r=3.9983;r=3.8553)。结论 190A/G(GG)、C242T(TT)基因型和吸烟是NAFLD的易患因素,基因多态性与吸烟的交互作用增加了NAFLD的发病风险。
Objective To investigate the interaction between the C242T polymorphism of the CCR2 gene 190A / G and the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase p22phox subunit gene and smoking of monocyte chemotactic factor-1 (MCP-1) And non-alcoholic fatty liver disease (NAFLD) relationship. Methods A case-control study was conducted in peripheral blood leukocytes of 600 patients with NAFLD and 600 healthy controls, and PCR-RFLP was used to analyze the expression of MCP- 1 receptor CCR2 gene 190A / G and NADPH oxidase p22phox subunit gene C242T polymorphism. Results The genotype frequency of 190A / G (GG) and C242T (TT) genotypes were 50.17% and 50.00% in case group and 23.83% and 24.17% in control group respectively. There was significant difference between the two groups (χ2 = 88.8462, P = 0.0031; χ2 = 85.8100, P = 0.0039). The 190 A / G (GG) genotype had a significantly increased risk of NAFLD (OR = 3.2171, 95% CI 1.9351 to 5.2184). The risk of NAFLD was also significantly increased in the C242T (TT) genotype (OR = 3.1379, 95% CI 1.7973-5.2362). The co-analysis of gene mutations found that the frequencies of 190A / G (GG) / C242T (TT) genotypes were 39.67% and 13.00% in the NAFLD group and the control group, respectively, and the difference was statistically significant (χ2 = 118.3021, P = 0.0017). The 190 A / G (GG) / C242T (TT) genotype had a significantly increased risk of NAFLD (OR = 5.0211, 95% CI 3.1853-7.7926). The smoking prevalence in case group was significantly higher than that in control group (χ2 = 92.2234, P = 0.0025). The risk of NAFLD was significantly increased in smokers (OR = 3.3032, 95% CI 1.9147-5.753) C242T (TT) genotype had interaction (r = 3.9983; r = 3.8553). Conclusion The genotypes of 190A / G (GG), C242T (TT) and smoking are risk factors for NAFLD. The interaction between genetic polymorphisms and smoking increases the risk of NAFLD.