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目的 探讨协同刺激分子在实验性变态反应性神经炎 (EAN)发病中的作用及雷公藤多甙的影响。方法 用兔坐骨神经匀浆免疫小鼠建立EAN模型 ,雷公藤多甙 (TWP)灌胃治疗 ,观察小鼠发病情况和病理改变 ;通过流式细胞计检测小鼠外周血淋巴细胞CD2 8、CTLA 4、B7 1、B7 2蛋白的表达 ;用RT PCR检测外周血淋巴细胞B7 1和B7 2mRNA的表达。结果 EAN小鼠外周血淋巴细胞上协同刺激分子CD2 8、CTLA 4、B7 1、B7 2蛋白的表达水平明显增高 ,B7 1和B7 2mRNA表达与蛋白的增加相平行。雷公藤多甙治疗组的发病率及病变程度均明显降低 ,同时伴随CD2 8、B7 1、B7 2蛋白的表达及B7 1、B7 2、mRNA表达的水平降低。结论 协同刺激分子的表达对T细胞活化起重要作用 ;雷公藤多甙能减轻ENA的病变程度 ,可能与抑制了B7 1及B7 2的基因转录或转录以上环节和抑制了CD2 8翻译或翻译以上环节有关。
Objective To investigate the role of costimulatory molecules in the pathogenesis of experimental allergic neuritis (EAN) and the effects of tripterygium glycosides. Methods Rabbit sciatic nerve homogenate was used to immunize mice to establish EAN model. Tripterygium wilfordii glycosides (TWP) were intragastrically administered to observe the pathogenesis and pathological changes of mice. CD28 and CTLA 4 of peripheral blood lymphocytes were detected by flow cytometry , B7 1, B7 2 protein expression; RT-PCR detection of peripheral blood lymphocytes B7 1 and B7 2 mRNA expression. Results The expression of costimulatory molecules CD28, CTLA 4, B7 1 and B7 2 on peripheral blood lymphocytes of EAN mice was significantly increased, and the expression of B7 1 and B7 2 mRNA was parallel to the increase of proteins. Tripterygium glycosides treatment group incidence and severity were significantly lower, accompanied by CD2 8, B7 1, B7 2 protein expression and B7 1, B7 2, mRNA levels decreased. Conclusions The expression of costimulatory molecules plays an important role in the activation of T cells. Tripterygium glycosides can reduce the severity of ENA, and may be related to the inhibition of the transcription and transcription of B7 1 and B7 2 genes and the up-regulation of CD8 translation or translation Related links.