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目的建立在大型哺乳动物-猪体内植入电子耳蜗的方法,观察电子耳蜗植入前后听功能变化。方法荣昌猪6只,雌雄不限,40-45日龄,体重8~12Kg,均选自重庆畜牧科学院养猪研究所。分为听力正常组(Mitf+/+),与突变耳聋组(Mitf-/-),每组3只。在全麻下进行电子耳蜗植入术。于手术前,手术后即刻以及手术后1周记录听性脑干反应(Auditory Brainstem Response,ABR),和电刺激脑干诱发电位(Electrical Auditory Brainstem Response,EABR);头颅X片观察电极植入位置。结果 6只动物电子耳蜗植入手术成功,耳蜗电极位置正确,在耳蜗内盘绕1.5~1.75圈。电子耳蜗植入即刻,手术侧(右耳)各频率ABR阈值在120 dB SPL无法引出;手术后7天,手术侧(右耳),低频ABR阈值在100dB SPL左右,高频在120 dB SPL仍无法引出。听力正常荣昌猪组(Mitf+/+),电子耳蜗植入手术即刻及一周后EABR阈值在90CL左右,明显低于突变耳聋荣昌猪组(Mitf-/-)的190CL。结论本研究建立的荣昌猪电子耳蜗植入方法确实可行,通过植入电极进行EABR测试方法设计合理。为更加直接地研究电子耳蜗植入设备在体内的工作状态和各项数据,研究电极植入后耳蜗的生理病理改变创造了条件。
Objective To establish a method of implanting cochlear implants in large mammals and pigs and observe the changes of hearing function before and after cochlear implantation. Methods Rongchang pig 6, male or female, 40-45 days old, weighing 8 ~ 12Kg, were selected from the Institute of Pigs, Chongqing Animal Husbandry Institute. Divided into normal hearing group (Mitf + / +), and sudden deafness group (Mitf - / -), 3 in each group. Cochlear implant under general anesthesia. Auditory Brainstem Response (ABR) and Electrical Auditory Brainstem Response (EABR) were recorded before surgery, immediately after surgery and 1 week after surgery. The locations of the electrodes . Results Six cochlear implants were successfully operated. The cochlear electrodes were correctly positioned and wound around the cochlea for 1.5-1.75 turns. Immediately after cochlear implantation, the ABR threshold of each side of the operation side (right ear) could not be extracted at 120 dB SPL. On the 7th day after operation, the ABR threshold of the operation side (right ear) and the low frequency was about 100 dB SPL and the high frequency was 120 dB SPL Unable to lead In normal hearing Rongchang pig group (Mitf + / +), the threshold of EABR immediately after and one week after cochlear implantation was about 90CL, which was significantly lower than 190CL of Mitchain - mutant deafness group (Mitf - / -). Conclusion The method of implanting cochlear implants in Rongchang pigs established in this study is indeed feasible. The design of EABR test by implanting electrodes is reasonable. In order to study the working status and data of the cochlear implant device in the body more directly, it is necessary to study the physiological and pathological changes of the cochlea after electrode implantation.