论文部分内容阅读
目的:建立苹果酸舒尼替尼脂质体含量及包封率的测定方法。方法:采用可见分光光度法测定苹果酸舒尼替尼的含量。阳离子交换树脂法分离游离药物,分光光度法测定脂质体中苹果酸舒尼替尼的包封率。结果:苹果酸舒尼替尼在430 nm处有最大吸收,4.0～15.0μg.mL-1范围内线性关系良好(r=0.9998,n=7);苹果酸舒尼替尼脂质体的平均包封率为91.89%。结论:所用方法简便、准确,可用于苹果酸舒尼替尼脂质体的含量及包封率测定。
OBJECTIVE: To establish a method for the determination of sunitinib malate liposome content and entrapment efficiency. Methods: The content of sunitinib malate was determined by visible spectrophotometry. Cation exchange resin separation of free drugs, spectrophotometric determination of encapsulation efficiency of sunitinib malate encapsulation efficiency. RESULTS: Sunitinib malate had the highest absorbance at 430 nm and good linearity in the range of 4.0 ~ 15.0 μg.mL-1 (r = 0.9998, n = 7). The average of sunitinib malate liposomes Encapsulation rate of 91.89%. Conclusion: The method is simple and accurate and can be used for the determination of encapsulation efficiency and encapsulation efficiency of sunitinib malate liposomes.