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为进一步挖掘利用高品质品系NM03102的优异纤维品质性状的基因,利用陆地棉鲁棉研21作为母本、NM03102为父本构建了F2和F2∶3分离群体。通过7892对SSR引物对亲本进行筛选,获得222对多态性引物,进一步对195个F2群体单株分析得到242个标记位点。其中,182个标记位点连锁构建37个连锁群,共覆盖1661.6 cM,每个连锁群平均包含4.9个标记位点,标记间平均相距9.1 cM,其中35个连锁群被定位到了20条染色体上。利用F2和F2∶3纤维品质数据,通过复合区间作图法,共检测到20个纤维品质性状QTL。其中,1个纤维强度的QTL和1个纤维整齐度的QTL与已有的报道一致,1个纤维强度的QTL和1个麦克隆值的QTL在两世代中稳定存在,这为标记辅助选择奠定了基础。
In order to further exploit the genes of excellent fiber quality traits using high-quality line NM03102, F2 and F2: 3 segregation populations were constructed by using cotton cultivar Lumianyan 21 as the female parent and NM03102 as the male parent. A total of 222 pairs of polymorphic primers were screened by 7892 pairs of SSR primers and 242 markers were further analyzed in 195 F2 populations. Among them, 182 marker loci were linked to construct 37 linkage groups, covering a total of 1661.6 cM. Each linkage group contained an average of 4.9 marker loci with an average distance of 9.1 cM between markers, of which 35 linkage groups were mapped to 20 chromosomes . A total of 20 QTLs for fiber quality traits were detected by composite interval mapping using F2 and F2: 3 fiber quality data. Among them, one QTL for fiber strength and one QTL for fiber uniformity were consistent with the previous reports that one QTL for fiber strength and one QTL for wheat clone were stable in two generations, which laid the foundation for marker-assisted selection The foundation.