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目的探讨不同条件的微波辐射对大鼠远后期学习和记忆功能、氨基酸类神经递质及海马组织结构影响。方法采用105只二级雄性Wistar大鼠,以平均功率密度的不同随机分为0 m W/cm2组、5 m W/cm2组和10 m W/cm2组,每组35只,辐射时间为6 min。于辐射后1 m、3 m、6 m、9 m、12 m和18 m,采用Morris水迷宫检测大鼠的学习和记忆功能;采用高效液相色谱检测大鼠海马组织中氨基酸类神经递质;采用光镜和电镜观察海马组织结构及超微结构。结果与0 m W/cm2组比较,5 m W/cm2组大鼠学习和记忆能力于辐射后1~18 m未见明显改变;10 m W/cm2组大鼠于辐射后1 m、3 m和18 m表现为平均逃避潜伏期明显延长。5 m W/cm2和10 m W/cm2组大鼠海马组织中4种氨基酸以降低性改变为主。5 m W/cm2组海马组织结构及超微结构未见明显改变;10 m W/cm2组于辐射后1 m海马神经元核固缩深染,血管周间隙增宽,后呈恢复趋势,但未完全恢复至正常水平。结论 5 m W/cm2微波辐射对大鼠远后期学习和记忆功能和海马组织结构无明显影响;10 m W/cm2微波辐射可致大鼠远后期学习和记忆功能下降,氨基酸类神经递质代谢紊乱及海马组织结构损伤。
Objective To investigate the effects of microwave irradiation on learning and memory function, amino acid neurotransmitters and hippocampal tissue in the late-stage rats. Methods 105 male Wistar rats were randomly divided into 0 mW / cm2 group, 5 mW / cm2 group and 10 mW / cm2 group, with 35 rats in each group. The average irradiation time was 6 min. Morris water maze was used to detect the learning and memory function of rats at 1 m, 3 m, 6 m, 9 m, 12 m and 18 m after radiation. Amino acid neurotransmitters The structure and ultrastructure of hippocampus were observed by light microscope and electron microscope. Results Compared with 0 m W / cm2 group, the learning and memory abilities of rats in 5 mW / cm2 group did not change significantly from 1 to 18 m after irradiation; And 18 m showed that the average escape latency was significantly prolonged. Four kinds of amino acids in the hippocampus of 5 mW / cm2 and 10 mW / cm2 groups were mainly decreased. At 5 mW / cm2, there was no significant change in the structure and ultrastructure of the hippocampus. In the 10 mW / cm2 group, the nuclei of the hippocampus became darker at 1 m after radiation and broadened the perivascular space, Not fully restored to normal levels. Conclusion Microwave radiation at 5 mW / cm2 has no significant effect on learning and memory function and hippocampal tissue structure in rats. Microwave radiation at 10 mW / cm2 can decrease the learning and memory ability of rats in the late stage, and the amino acid neurotransmitter metabolism Disorders and structural damage in the hippocampus.