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目的:研究碱性成纤维细胞生长因子(bFGF)对人卵巢癌CAOV3细胞细胞周期调节蛋白cyclinD1及GADD153表达的影响,探讨bFGF促进人卵巢癌CAOV3细胞增殖、抑制凋亡的信号机制。方法:利用无血清饥饿诱导卵巢癌CAOV3细胞凋亡。分为对照组、bFGF组。分别应用MTT、流式细胞术、琼脂糖凝胶电泳观察25、50、75μg/L bFGF对CAOV3细胞增殖率、细胞周期及细胞凋亡的影响。利用Western blotting检测bFGF对CA-OV3细胞cyclin D1、GADD153以及转录因子(c-Fos、c-Jun)表达的影响。结果:与对照组相比,bFGF呈剂量依赖性加速CAOV3细胞细胞周期进程,促进细胞增殖,抑制饥饿诱导的凋亡(P<0.01);呈时间依赖性促进cyclinD1、c-Fos、c-Jun,抑制GADD153蛋白表达(P<0.01)。结论:bFGF可能通过上调cyclin D1、c-Fos、c-Jun,下调GADD153表达促进细胞增殖,抑制饥饿诱导的卵巢癌CAOV3细胞凋亡。
OBJECTIVE: To study the effect of basic fibroblast growth factor (bFGF) on the expression of cyclinD1 and GADD153 in human ovarian cancer cell line CAOV3, and to explore the signal mechanism of bFGF promoting proliferation and inhibiting apoptosis of human ovarian cancer cell line CAOV3. Methods: Apoptosis of ovarian cancer cell line CAOV3 was induced by serum free starvation. Divided into control group, bFGF group. MTT, flow cytometry and agarose gel electrophoresis were used to observe the effects of 25, 50 and 75μg / L bFGF on the proliferation, cell cycle and apoptosis of CAOV3 cells. The effect of bFGF on the expression of cyclin D1, GADD153 and c-Fos (c-Jun) in CA-OV3 cells was detected by Western blotting. Results: Compared with the control group, bFGF accelerated the cell cycle progression of CAOV3 cells in a dose-dependent manner, promoted cell proliferation and inhibited starvation-induced apoptosis (P <0.01), and promoted the expression of cyclinD1, c-Fos and c-Jun , Inhibited GADD153 protein expression (P <0.01). CONCLUSION: bFGF may up-regulate cyclin D1, c-Fos, c-Jun and down-regulate the expression of GADD153 to promote cell proliferation and inhibit starvation-induced apoptosis of ovarian cancer CAOV3 cells.