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目的:探讨巢蛋白和血管内皮生长因子(VEGF)在脑胶质瘤患者中的表达。方法:应用免疫组织化学法检测低级别人脑胶质瘤20例(低级别组)、高级别人脑胶质瘤23例(高级别组)和正常人30例(对照组)脑组织的巢蛋白表达及用酶联免疫吸附法检测血清VEGF在各组人群中的表达水平。结果:人脑组织巢蛋白的阳性细胞数在对照组、低级别组和高级别组分别为5.15±0.37、8.20±1.32和9.65±1.47,三组之间差异均有统计学意义(P﹤0.05);血清VEGF浓度在对照组、低级别组和高级别组分别为(134.05±21.57)pg/ml、(411.75±11.29)pg/ml和(456.23±18.34)pg/ml,三组之间差异均有统计学意义(P﹤0.05);并且脑胶质瘤患者血清VEGF浓度和其脑组织nestin的表达成正相关(r=0.363,P<0.05)。结论:人脑胶质瘤的分级越高,nestin和VEGF的表达就越强,两者均可成为判断脑胶质瘤患者预后的指标。
Objective: To investigate the expression of nestin and vascular endothelial growth factor (VEGF) in glioma patients. Methods: Immunohistochemistry was used to detect the expression of nestin in 20 cases of low grade human gliomas (low grade group), 23 cases of high grade gliomas (high grade group) and 30 normal controls (control group) Serum VEGF expression levels in each group were detected by enzyme-linked immunosorbent assay. Results: The positive cells of nestin in human brain tissue were 5.15 ± 0.37,8.20 ± 1.32 and 9.65 ± 1.47 in the control group, the low-grade group and the high-grade group, respectively, with significant difference between the three groups (P <0.05 ). Serum VEGF concentration was (134.05 ± 21.57) pg / ml, (411.75 ± 11.29) pg / ml and (456.23 ± 18.34) pg / ml respectively in the control group, low-grade group and high-grade group. (P <0.05). The serum VEGF concentration in brain gliomas was positively correlated with the expression of nestin (r = 0.363, P <0.05). Conclusion: The higher the grade of human glioma, the stronger the expression of nestin and VEGF, both of which can be used as indicators to judge the prognosis of patients with glioma.