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土壤全氮量是土壤的重要肥力指标之一,土壤全氮量的测定对农业生产和科学研究都有重要意义。百余年来沿用的杜马氏法和开氏定氮法无疑是经典的方法,但手续冗长,设备复杂。利用酸性偶氮染料上的磺酸基在酸性条件下与蛋白质上的碱性氨基酸如赖氨酸、精氨酸、组氨酸及某些碱性基团如胍基、咪唑基等形式沉淀,测定剩余染料浓度,换算为蛋白质含量,是近代发展的一种快速方法。用该法快
Soil total nitrogen is one of the important soil fertility indicators, soil total nitrogen determination of agricultural production and scientific research are of great significance. The Duma’s method and the Kjeldahl method, which have been used for more than 100 years, are undoubtedly the classic method, but the procedures are lengthy and the equipment is complicated. The sulfonic acid group on the acidic azo dye is precipitated under acidic conditions with basic amino acids such as lysine, arginine, histidine and some basic groups such as guanidino and imidazolium on the protein, Determination of the remaining dye concentration, converted to protein content, is a rapid development of modern methods. Use this method fast