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输精管吻合术后精子重现率为60～95%。不过尽管手术在技术上非常成功,但继后的妊娠率仅为30～70%。输精管切除或吻合术后的继发性不育认为与精浆中的抗精子抗体有关。抗精子抗体可阻碍精子穿透宫颈粘液并降低其受精能力。不过对这一观点的意见并不统一。Parslow等(1983)曾报告精浆抗体滴度达1∶16时仍可妊娠,认为与输精管吻合术相关的抗精子抗体可能根本不同于发生在其它生育力低下男子中的抗体。本研究采用间接免疫珠法(IBT)检测了作输精管吻合术后男子精浆中的IgA与IgG型抗精子抗体。旨在①对比IBT与玻盘凝集试验的结果;②计算输精管吻合术前后IgA与IgG型抗精子抗体的发生率;③确定
Vasectomy sperm reproductive rate of 60 to 95%. However, although the operation was technically very successful, the subsequent pregnancy rate was only 30-70%. Secondary sterility after vasectomy or anastomosis is considered to be associated with anti-sperm antibodies in seminal plasma. Anti-sperm antibodies can hinder sperm penetration of cervical mucus and reduce their ability to fertilize. However, opinions on this view are not uniform. Parslow et al. (1983) reported that seminal plasma antibody titer of 1: 16 was still measurable, suggesting that anti-sperm antibodies associated with vas deferens anastomoses may be fundamentally different from those occurring in other fertile men. In this study, indirect immunobead beads (IBT) were used to detect anti-sperm IgA and IgG anti-sperm antibodies in seminal plasma of anastomosis patients after vasectomy. The aim is to compare the results of IBT with glass plate agglutination test, calculate the incidence of anti-sperm antibodies of IgA and IgG before and after vasectomy,