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目的 探讨肾癌组织中微血管形成与肿瘤细胞凋亡的关系。方法 应用免疫组织化学方法对 51例肾癌组织中血管内皮细胞的第Ⅷ因子抗原进行染色 ,并计数肿瘤的微血管 ;应用原位DNA片段末端标记法检测肾癌组织细胞凋亡状态。结果 肾细胞癌微血管密度在Ⅰ、Ⅱ、Ⅲ级肿瘤中分别为 49.3± 2 5.3、54.0± 2 8.8、94.9± 38.7;在Ⅰ、Ⅱ、Ⅲ Ⅳ期肿瘤中分别为 45.2± 2 1 .2、68.5±32 .6、99.5± 43.7,其密度在肿瘤的临床分期及病理分级间差异具有显著性 (P <0 .0 5)。肾细胞癌微血管密度与肿瘤细胞的凋亡指数呈负相关 (rs=- 0 .61 8,P <0 .0 0 1 ) ,但与肿瘤的Ki67标记指数无关(rs=0 .0 96,P >0 .0 5)。结论 在肾细胞癌发展过程中 ,微血管的形成可抑制肿瘤细胞的凋亡 ,而促进肿瘤的恶性进展
Objective To investigate the relationship between microvessel formation and tumor cell apoptosis in renal cell carcinoma. Methods Immunohistochemistry was used to stain the factor Ⅷ antigen of vascular endothelial cells in 51 cases of renal cell carcinoma and to count the microvessels of tumor. The apoptotic status of renal cell carcinoma was detected by in situ DNA fragment end labeling. Results The microvessel density of renal cell carcinoma was 49.3 ± 2 5.3, 54.0 ± 8.88 and 94.9 ± 38.7 respectively in grade Ⅰ, Ⅱ and Ⅲ tumors, and 45.2 ± 21.2 in stage Ⅰ, Ⅱ and Ⅲ Ⅳ tumors, 68.5 ± 32.6,99.5 ± 43.7, the density of tumor in the clinical stage and pathological grade difference was significant (P <0.05). The microvessel density of renal cell carcinoma was negatively correlated with the apoptosis index of tumor cells (rs = - 0.61 8, P <0.01), but not with the Ki67 labeling index (rs = 0.96, P > 0 .0 5). Conclusions In the development of renal cell carcinoma, the formation of microvessels can inhibit the apoptosis of tumor cells and promote the malignant progression of the tumor