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目的观察二母颗粒对C57BL/6小鼠体内Lewis肺癌生长、转移以及血管生成的影响及其作用机制。方法制备C57BL/6小鼠Lewis肺癌模型,随机分为二母颗粒高、中、低(3、2、1 g.kg-1生药)剂量组、环磷酰胺组、沙利度胺组、模型组。分别给予相应药物后,观察各组小鼠的肿瘤生长情况及肺转移发生率,采用免疫组化方法观察瘤内血管内皮细胞生长因子(VEGF)和微血管密度(MVD)的表达。结果二母颗粒高、中、低剂量组连续ig给药14 d,对小鼠Lewis肺癌的抑瘤率分别为30.80%、10.63%、0。其中,二母颗粒高剂量组的瘤重、抑瘤率、VEGF及MVD的表达与模型组比较均有显著差异(P<0.05)。结论二母颗粒具有抑制小鼠Lewis肺癌的作用,其作用机制可能与抑制瘤内VEGF和MVD的生成有关。
Objective To observe the effects of Ermu granule on the growth, metastasis and angiogenesis of Lewis lung carcinoma in C57BL / 6 mice and its mechanism. Methods Lewis lung carcinoma model of C57BL / 6 mice was established and randomly divided into two groups: high dose, middle dose and low dose (3,2,1 g.kg-1 crude drug), cyclophosphamide group, thalidomide group, model group. After given the corresponding drugs, the growth of the mice in each group and the incidence of lung metastasis were observed. The expression of VEGF and MVD in the tumor were observed by immunohistochemistry. Results The inhibitory rates of two drugs on mice with Lewis lung cancer were 30.80% and 10.63%, respectively, after continuous ig administration for 14 d in high, medium and low dose groups. Among them, the tumor weight, tumor inhibition rate, the expression of VEGF and MVD in Ermu Granule high-dose group were significantly different from those in model group (P <0.05). Conclusion Er-miao granules can inhibit the Lewis lung cancer in mice, and its mechanism may be related to the inhibition of the formation of VEGF and MVD in the tumor.