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采用不同浓度的NaCl(A)和NaCl+NaHCO3(B)盐溶液对当地选育出的虉草新品系(Phalaris arundinacea)进行3周盐胁迫处理,通过测定茎叶生物量、根系生物量、茎叶相对含水率、叶片电导率以及丙二醛、叶绿素、脯氨酸含量等,探讨2种盐胁迫对草苗期生理与生长的影响.结果表明,随着盐浓度的升高虉草茎叶生物量、根系生物量、茎叶相对含水率、叶绿素含量逐渐降低,茎叶生物量下降幅度大于根系生物量;叶片电导率、丙二醛含量和脯氨酸含量随盐浓度升高而上升.当盐浓度为100mmol/L时,A、B组处理叶片电导率、游离脯氨酸含量分别比对照高34.5%、25.16%和56.85%、45.34%,差异显著(P<0.05),而茎叶生物量和叶绿素含量分别比对照低13.2%、19.01%和24.43%、23.9%,差异极显著(P<0.01).NaCl+NaHCO3混合盐处理对虉草苗期生长的影响大于NaCl处理,表明草对碱性盐胁迫较敏感.
The salt solution of Phalaris arundinacea, which was bred locally, was treated with salt solution of NaCl (A) and NaCl + NaHCO3 (B) at different concentrations for three weeks. The leaf biomass, root biomass, Leaf relative water content, leaf electrical conductivity, MDA content, chlorophyll content and proline content in the seedling stage were investigated to investigate the effects of two kinds of salt stress on the growth and physiology of grass seedlings. The results showed that with the increase of salt concentration, The biomass, root biomass, relative water content of stems and leaves, chlorophyll content decreased gradually, and the biomass of stems and leaves decreased more than that of root biomass. The leaf conductivity, malondialdehyde content and proline content increased with the increase of salt concentration. When the salt concentration was 100 mmol / L, the leaf electrical conductivity and free proline content in leaves of treatments A and B were 34.5%, 25.16% and 56.85%, 45.34% higher than that of the control respectively (P <0.05) The biomass and chlorophyll content were 13.2%, 19.01% and 24.43%, 23.9% lower than the control respectively (P <0.01) .The effect of NaCl + NaHCO3 mixed salt treatment on seedling growth was greater than that of NaCl treatment, Alkaline salt stress is more sensitive.