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目的 分析RANTES启动子区等位基因在中国维吾尔族和汉族健康人和HIV 1感染者中的分布特点 ,以期阐明RANTES基因突变型和人类免疫缺陷病毒(HIV 1 )感染在不同民族人群的关系。方法 利用PCR RFLP法对维吾尔族和汉族HIV 1感染者和健康人群的 86 5份样品的RANTES 4 0 3、 2 8等位基因进行检测。结果 两个位点在维吾尔族和汉族人群均有较高的等位基因频率 ,分布基本一致 ;RANTES等位基因具有 6个基因表型 ,分别为AC/AC、AC/AG、AC/GC、AG/GC、GC/GC和AG/AG ;从单倍型看 ,汉族和维吾尔族均以GC为最高 ,占 6 2 7% ,AC分别为 2 8 7%和 30 4 % ,AG分别为 8 6 %和 6 8%。但是从单个位点看 ,HIV感染者和健康人、吸毒者差异均无统计学意义(P >0 0 5 ) ;从基因表型分析 ,与AC/AC比较 ,AC/AG、AC/GC、AG/GC、GC/GC的OR值显示都有不同程度的关联(OR =0 33～0 81 )。结论 汉族和维吾尔族中RANTES启动子区 4 0 3/ 2 8均有较高的突变频率 ;与AC/AC比较 ,AC/AG、AC/GC、AG/GC、GC/GC的OR值显示都有不同程度的保护作用
Objective To analyze the distribution of RANTES promoter alleles in healthy Uygur and Han Chinese and HIV-1 infected persons in order to clarify the relationship between the RANTES gene mutation and human immunodeficiency virus (HIV 1) infection in different ethnic groups. Methods The RANTES 403 and 88 alleles of 865 samples from Uygur and Han HIV-1 infected and healthy population were detected by PCR-RFLP. Results The two loci had higher allele frequency in Uygur and Han nationality population, and their distribution was basically the same. The RANTES allele had 6 phenotypes, which were AC / AC, AC / AG, AC / GC, AG / GC, GC / GC and AG / AG. From the haplotypes, both Han and Uygur populations had the highest GC (62.7%) with ACs of 28.7% and 30.4% 6% and 68%. However, there was no significant difference between HIV-infected and healthy people and drug addicts from a single locus (P> 0.05). According to the phenotypic analysis, AC / AC, AC / GC, The OR values of AG / GC and GC / GC showed different degrees of correlation (OR = 0 33 ~ 0 81). CONCLUSION: The frequency of mutation in the RANTES promoter region of Han and Uygur nationality is higher than that of the wild type and the Uygur nationality respectively. The OR of AC / AG, AC / GC, AG / GC and GC / GC is higher than that of AC / AC Have different degrees of protection