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40年代Coon’s等用荧光抗体法为免疫病理学的研究开辟了道路。60年代酶免疫技术的使用,特别是70年代Sternberger等建立的过氧化物酶—抗过氧化物酶法(PAP),极大地推动了免疫病理学的发展。PAP法敏感、简便,不需复杂的仪器,且能应用于常规固定的石蜡切片,因而深受病理学家的欢迎,但有时亦可因组织中抗原物质丢失,出现假阴性。70年代末Guesdon等将亲和素—生物素系统(Avidin-biotin systems)应用于免疫酶技术,80年代Hstu等进一步吸取了Guesdon的亲和素—生物素架桥法(Bridged avidin-biotin,BRAB)和标记
In the 1940s, Coon’s et al. Opened the way for the study of immunopathology with fluorescent antibody. The use of enzyme immunoassay techniques in the 1960s, and in particular the peroxidase-anti-peroxidase (PAP) method established by Sternberger et al. In the 1970s, greatly facilitated the development of immunopathology. PAP is sensitive and easy to use, does not require complicated instruments, and can be applied to routinely fixed paraffin sections, which are very popular with pathologists. However, PAP can sometimes be false negative due to the loss of antigenic substances in tissues. In the late 1970s, Guesdon et al. Applied Avidin-biotin systems to immune enzyme technology. In the 1980s Hstu et al further absorbed Guesdon’s avidin-biotin (BRAB ) And mark