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建立1种制备性分离南方红豆杉叶氯仿萃余相中黄酮类化合物的方法。将氯仿萃取南方红豆杉叶醇提物剩余相用AB-8型大孔树脂层析柱进行初分离,收集浓缩样品,用聚酰胺层析柱分离纯化浓缩液,硝酸钠-硝酸铝显色,紫外分光光度法定量测定总黄酮含量。优化的AB-8型大孔树脂分离紫杉黄酮工艺条件为:样品进样体积60 mL(1 BV),解吸液为80%乙醇,解吸体积180 mL,在此条件下浸膏紫杉黄酮含量达到20.65%;优化的聚酰胺层析柱分离紫杉黄酮化合物工艺条件为:样品进样体积30 mL(1 BV),解吸液为70%乙醇,解吸液体积120 mL,在此条件下浸膏黄酮含量达63.05%。红豆杉叶醇提液的氯仿剩余相中总黄酮含量为2.83%。AB-8型大孔树脂层析与聚酰胺层析联合分离使总黄酮百分含量提高60.22倍。
A method for the preparative separation of flavonoids from the chloroform remnants of Taxus chinensis var. Chinensis was established. The residual phase of ethanol extract from Taxus chinensis var.mairenensis leaves was initially separated by column chromatography on AB-8 macroporous resin. The concentrated samples were collected and purified by polyamide column chromatography. The sodium nitrate- Determination of Total Flavonoids by UV Spectrophotometry. The optimal conditions for the separation of taxanes from AB-8 macroporous resin were as follows: the sample injection volume was 60 mL (1 BV), the desorption solution was 80% ethanol and the desorption volume was 180 mL. Under this condition, Reached 20.65%. The optimum conditions for the separation of taxanes from polyamide columns were as follows: the sample injection volume was 30 mL (1 BV), the desorption solution was 70% ethanol and the desorption solution volume was 120 mL. Under these conditions, the extract Flavonoids content of 63.05%. The content of total flavonoids in the residual phase of the chloroform extract of yew leaf was 2.83%. AB-8 macroporous resin chromatography and polyamide chromatography combined to increase the percentage of total flavonoids 60.22 times.