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目的探讨安徽省手足口病患儿中肠道病毒71型(human enterovirus 71,HEV71)的分子流行病学特征。方法对2009-2012年HEV71所致手足口病患儿的19份咽拭子标本进行HEV71病毒VP1区全基因核苷酸序列测定,其中1份标本来自河南信阳患儿,其余均来自安徽省辖区患儿。所得序列与国内外HEV71毒株的核苷酸序列做比对分析,构建进化树。结果 19株病毒核酸VP1区全基因均为891个核苷酸,编码297个氨基酸。所得序列与A、B、C基因型的核苷酸同源性分别为82.6%~83.6%、83.3%~85.5%、86.7%~98.9%;氨基酸同源性分别为96.0%~97.0%、96.0%~97.7%、97.0%~100.0%。结论六安市2009-2012年HEV71流行株在种系进化上与我国大陆近几年分离的毒株具有较高的同源性(94.6%~98.9%),同属于C4a基因亚型,与中国大陆前几年分离株C4b基因亚型较近。
Objective To investigate the molecular epidemiological characteristics of human enterovirus 71 (HEV71) in children with hand-foot-mouth disease in Anhui Province. Methods Nineteen throat swab specimens of HEV71-induced hand-foot-mouth disease in 2009-2012 were tested for the nucleotide sequence of HEV71 VP1 gene, of which 1 was from Xinyang, Henan Province and the others were from Anhui Province Children. The obtained sequence was compared with the nucleotide sequence of HEV71 strain at home and abroad to construct the phylogenetic tree. Results The 19 strains of viral nucleic acid VP1 region of all genes are 891 nucleotides, encoding 297 amino acids. The nucleotide homologies of the obtained sequences to A, B and C genotypes were 82.6% -83.6%, 83.3% -85.5% and 86.7% -98.9%, respectively. The amino acid homologies were 96.0% -97.0%, 96.0% % ~ 97.7%, 97.0% ~ 100.0%. Conclusion The epidemic strains of HEV71 from 2009 to 2012 in Lu’an City have a high homology (94.6% ~ 98.9%) with the strains isolated in recent years in mainland China, belonging to the C4a subtype, A few years ago the isolates of the isolate C4b genotypes in China were close.