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IL-7是一种分子量为20KDa 的糖蛋白,主要由骨髓和胸腺的基质细胞产生,具有诱导前B 细胞和T 细胞增殖的作用,诱导T 细胞IL-2受体α链、转铁蛋白受体和T 细胞的活化标志4F2的表达,是一种除IL-2、IL-4之外的新的T 细胞生长因子。本文采用健康成人的PBMC 或分离不同的淋巴细胞群,在加有人AB 型血清的PRMI-1640中进行培养,分别在IL-2、IL-7、IL-4及其不同组合的刺激下观察LAK 细胞的产生和活性。采用流式细
IL-7 is a glycoprotein with a molecular weight of 20 kDa, which is mainly produced by stromal cells of bone marrow and thymus. It induces the proliferation of pre-B cells and T cells, induces the IL-2 receptor α chain of T cells, The expression of 4F2, an activating marker of T cell and T cell, is a novel T cell growth factor other than IL-2 and IL-4. In this study, healthy adult PBMCs or different lymphocyte populations were isolated and cultured in PRMI-1640 supplemented with human AB serum. LAKs, IL-7, IL-4 and their different combinations of stimuli were observed LAK Cell production and activity. Use of thin flow