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实验用胚胎成纤维细胞单层培养模型,进行药物筛选。按病毒学方法制备细胞培养物。把细胞培养物(700,000～1,000,000个/ml)移入试管中,用橡皮塞塞紧,放入37℃恒温室中孵育。经4昼夜,在试管壁上生长一大片细胞,然后按1 ml 培养基加入0.1 ml 杀虫剂和保护药的混液,乐果和敌螨通剂量分别为7.810~(-7)ml/ml 和5.0·10~(-7)ml/ml,维生素 C、B_1、B_6和 B_(12)为 5 r/ml,叶酸和维生素 B_2为0.5 r/ml,蛋氨酸为50 r/ml,盐酸胆碱为20 r/ml.
Experimental embryo fibroblast monolayer culture model for drug screening. Cell cultures were prepared by virological methods. Cell cultures (700,000-1,000,000 / ml) were transferred to test tubes, stuffed with rubber stoppers and incubated in a 37 ° C incubator. After 4 days and nights, a large cell was grown on the wall of the test tube, and then 0.1 ml of the mixture of insecticide and protective drug was added to 1 ml of medium. The doses of dimethoate and propargyl were respectively 7.810-7 ml / ml And 5.0 · 10 ~ (-7) ml / ml respectively, 5 r / ml for vitamin C, B_1, B_6 and B_ (12), 0.5 r / ml for folic acid and vitamin B_2, 50 r / ml for methionine, 20 r / ml.