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应用不同浓度的Ｃ－ｍｙｃ反义寡核苷酸处理体外培养的经１０－８ｍｏｌ／Ｌ内皮素－１（ＥＴ－１）诱导的人肺动脉平滑肌细胞，通过细胞计数和３Ｈ－ＴｄＲ掺入值观察该反义核酸对细胞增殖及ＤＮＡ合成的抑制作用，应用斑点杂交法检测平滑肌细胞Ｃ－ｆｏｓ和Ｃ－ｍｙｃｍＲＮＡ的表达水平。结果表明，１０、２０、５０μｇ／ｍｌ反义寡核苷酸对细胞计数和３Ｈ－ＴｄＲ掺入值与对照组相比抑制率分别为１１．９％和１２．３％（Ｐ＜０．０５），２１．６％和１４．７％（Ｐ＜０．０１），３６．３％和２７．２％（Ｐ＜０．０１）；对Ｃ－ｍｙｃｍＲＮＡ表达抑制率分别为７１．３％（Ｐ＜０．０５），８０．７％（Ｐ＜０．０５），９２．５％（Ｐ＜０．０１）；５０μｇ／ｍｌ反义核酸对Ｃ－ｆｏｓｍＲＮＡ表达抑制率是７９．７％，１０、２０μｇ／ｍｌ组无抑制作用。Ｃ－ｍｙｃ反义核酸能显著抑制内皮素诱导的人肺动脉平滑肌细胞增殖，其作用系依赖反义核酸的序列特异性和剂量依赖。
Human pulmonary artery smooth muscle cells induced by 10-8 mol / L endothelin-1 (ET-1) cultured in vitro were treated with different concentrations of C-myc antisense oligonucleotide and observed by cell counting and 3H-TdR incorporation The antisense nucleic acid on cell proliferation and DNA synthesis inhibition, the use of dot blot hybridization detection of smooth muscle cells C-fos and C-mycmRNA expression levels. The results showed that the inhibitory rates of 10, 20, 50μg / ml antisense oligonucleotides on cell counts and 3H-TdR incorporation were 11.9% and 12.3%, respectively (P <0.05 ), 21.6% and 14.7% (P <0.01), 36.3% and 27.2% respectively (P <0.01), and the inhibitory rates of C-mycmRNA expression were 71.3% P <0.05), 80.7% (P <0.05), 92.5% (P <0.01). The inhibition rate of C-fos mRNA expression by antisense oligonucleotide was 79.7% 10,20μg / ml group did not inhibit. C-myc antisense nucleic acid can significantly inhibit endothelin-induced proliferation of human pulmonary artery smooth muscle cells, the role of antisense-type nucleic acid-dependent sequence-specific and dose-dependent.