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目的:研究表皮生长因子(EGF)对大鼠小肠缺血-再灌注(I-R)损伤后肠黏膜屏障的保护作用。方法:将36只大鼠随机分为假手术组(对照组),模型组(I-R组),大剂量预处理组(Pre-L组),小剂量预处理组(Pre-S),大剂量后处理组(Post-L)和小剂量后处理组(Post-S),每组各6只。于缺血30 min再灌注30 min时,分别取大鼠的血和小肠标本,检测小肠组织病理学评分(Chiu’s)和细胞增殖程度(Ki-67),同时检测血清中D-乳酸、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的浓度。结果:与对照组比,I-R组大鼠血清D-乳酸、TNF-α、IL-6水平和病理学Chiu’s评分明显升高,而细胞增殖程度无明显改变。与I-R组比,Pre-L、Pre-S和Post-L组的Chiu’s评分及Ki67显著升高;Pre-L组大鼠D-乳酸显著降低;TNF-α和IL-6在四个处理组均有明显降低。结论:EGF对大鼠小肠I-R损伤后肠黏膜屏障具有防护作用。预防性较大剂量使用可能预后更好。
Objective: To study the protective effect of epidermal growth factor (EGF) on intestinal mucosal barrier after intestinal ischemia-reperfusion (I-R) injury in rats. Methods: Thirty-six rats were randomly divided into sham operation group (control group), model group (IR group), high-dose pretreatment group (Pre-L group), low-dose pretreatment group Post-L group and Post-S group, 6 rats in each group. Blood and small intestine specimens were collected from the rats for 30 min after reperfusion for 30 min, respectively. Chiu’s and Ki-67 were detected, and serum levels of D-lactate, tumor necrosis Factor-α (TNF-α) and interleukin-6 (IL-6) concentrations. Results Compared with the control group, the levels of serum D-lactate, TNF-α, IL-6 and pathological Chiu’s score in I-R group were significantly higher than those in control group, but there was no significant change in the degree of cell proliferation. Compared with IR group, Chiu’s score and Ki67 in Pre-L, Pre-S and Post-L groups were significantly increased; D-lactate in Pre-L group was significantly decreased; TNF-α and IL-6 in the four treatment groups Have significantly reduced. Conclusion: EGF has a protective effect on intestinal mucosal barrier after I-R injury in rats. Prophylactic use of larger doses may be better prognosis.