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采用固相萃取(SPE)-超高效液相色谱-串联质谱法(UPLC-MS/MS)对酱卤鸡腿老汤中的杂环胺进行提取和检测。酱卤鸡腿的老汤样品经过超声提取,离心分离,固相萃取小柱富集,甲醇-氨水洗脱,最后经UPLC-MS/MS法检测分析。结果表明,10种杂环胺标准品在2~200μg/L线性范围良好,相关系数r>0.990,在添加水平10,20和50μg/L时回收率为55.6%~100.2%,相对标准偏差为1.4%~6.4%。SPE-UPLC-MS/MS法处理样品时间短,分析速度快,定量准确,能够应用于老汤中杂环胺的检测。
The heterocyclic amines were extracted and detected by SPE-UPLC-MS / MS. The samples of soup with sauce-cured chicken drumstick were extracted by ultrasonic, centrifuged, concentrated by solid-phase extraction cartridges and eluted with methanol-ammonia water, and finally analyzed by UPLC-MS / MS. The results showed that the calibration curve of 10 kinds of heterocyclic amine was good in the linear range of 2 ~ 200μg / L with the correlation coefficient r> 0.990. The recoveries were 55.6% ~ 100.2% at the levels of 10, 20 and 50μg / L. The relative standard deviations 1.4% ~ 6.4%. SPE-UPLC-MS / MS sample processing time is short, fast analysis, quantitative accuracy, can be applied to the detection of heterocyclic amine in Lao Tang.