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探讨鳖甲活性多肽的固相合成及对肝星状细胞(HSC-T6)增殖的抑制作用。根据鳖甲活性多肽的序列结构,采用固相方法对多肽进行全合成,并用MALDI-TOFMS质谱对合成多肽进行分析鉴定;肝星状细胞(HSC-T6)培养至对数生长期后,加入不同浓度合成多肽作用肝星状细胞株HSC-T6,采用MTS法分析HSC-T6生长的抑制作用;使用AnnexinV-FITC/PI法检测HSC凋亡率。结果显示通过固相合成可以得到与原序列结构一致的鳖甲合成多肽;鳖甲合成多肽浓度依赖性地抑制肝星状细胞增殖,并能显著提高肝星状细胞早期凋亡率。因此,可以成功地合成鳖甲活性多肽,且对肝星状细胞增殖有明显的抑制作用。
To explore the solid-phase synthesis of Biejia active peptides and its inhibitory effect on the proliferation of hepatic stellate cells (HSC-T6). According to the sequence structure of the active peptides of Biejiaju, the peptides were synthesized by solid-phase method and the synthesized peptides were identified by MALDI-TOF MS. After HSC-T6 cultured to logarithmic growth phase, The inhibitory effect of HSC-T6 on hepatic stellate cell line HSC-T6 was analyzed by MTS assay. The apoptosis rate of HSC was detected by Annexin V-FITC / PI assay. The results showed that the synthesized peptide of turtle shell could be obtained by solid-phase synthesis. The synthetic peptide of turtle shell inhibited the proliferation of hepatic stellate cells in a concentration-dependent manner and significantly increased the rate of early apoptosis of hepatic stellate cells. Therefore, we can successfully synthesize turtle shell active peptides, and inhibit hepatic stellate cell proliferation significantly.