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目的探讨重组人可溶性补体受体1型 SCR15-18片段(sCR1-SCR15-18)对心肌缺血再灌注的保护作用。方法 36只 SD 大鼠随机分为假手术(SO)组,缺血再灌注(I/R)组和 sCR1-SCR15-18(sCR1)保护组。建立急性心肌缺血再灌注模型,结扎冠状动脉前立即注射磷酸盐缓冲液(0.1 ml/100 g)或 sCR1-SCR15-18蛋白(15 mg/kg)。测定心肌梗塞面积,血清中乳酸脱氢酶(LDH)和肌酸激酶(CK),心肌组织髓过氧化物酶(MPO)活性,HE 染色观察心肌病理改变和免疫组织化学法检测 C3c。结果(1)心肌梗死面积:I/R 组为(22.9±3.0)%,sCR1保护组为(16.1±3.3)%(P<0.05)。(2)血清心肌酶 CK(U/L):I/R 组为3400.9±534.9,sCR1保护组为2532.5±597.1(P<0.05)。LDH(U/L):I/R 组为6572.0±476.3,sCR1保护组为5436.2±611.3(P<0.05)。(3)心肌组织 MPO 活性(U/g):I/R 组为1.12±0.13,sCR1保护组为0.81±0.14(P<0.05)。(4)心肌病理改变:I/R 组心肌有断裂、坏死,间质肿胀,出血及中性粒细胞浸润,sCR1保护组心肌的以上病理变化明显较 I/R 组减轻。(5)与 VR 组比 sCR1保护组梗死区心肌组织 C3c 的沉积减少。结论 sCR1-SCR15-18蛋白对大鼠急性心肌缺血再灌注损伤具有保护作用。
Objective To investigate the protective effect of recombinant human soluble complement receptor type 1 SCR15-18 fragment (sCR1-SCR15-18) on myocardial ischemia-reperfusion. Methods Thirty - six SD rats were randomly divided into sham operation (SO) group, ischemia / reperfusion (I / R) group and sCR1-SCR15-18 (sCR1) protection group. Acute myocardial ischemia-reperfusion model was established. Phosphate buffered saline (0.1 ml / 100 g) or sCR1-SCR15-18 protein (15 mg / kg) was injected immediately prior to ligation of the coronary arteries. Myocardial infarction area, LDH, CK and MPO activity in myocardium were determined. Pathological changes of myocardium were observed by HE staining and C3c was detected by immunohistochemistry. Results (1) The area of myocardial infarction was (22.9 ± 3.0)% in I / R group and (16.1 ± 3.3)% in sCR1 group (P <0.05). (2) Serum CK (U / L): 3400.9 ± 534.9 in I / R group and 2532.5 ± 597.1 in sCR1 protection group (P <0.05). LDH (U / L): 6572.0 ± 476.3 for I / R group and 5436.2 ± 611.3 for sCR1 protection group (P <0.05). (3) Myocardial MPO activity (U / g) was 1.12 ± 0.13 in I / R group and 0.81 ± 0.14 in sCR1 protection group (P <0.05). (4) Myocardial pathological changes: Myocardium in I / R group had rupture, necrosis, interstitial swelling, hemorrhage and neutrophil infiltration. The above pathological changes of myocardium in sCR1 protective group were significantly relieved compared with I / R group. (5) Compared with VR group, the deposition of C3c in myocardial infarction area in sCR1-protected group decreased. Conclusion sCR1-SCR15-18 protein has a protective effect on acute myocardial ischemia-reperfusion injury in rats.