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采用碘-碘化钾法、氯化三苯基四氮唑(TTC)法及离体萌发法对花粉活力进行了室内测定,并与大田测定结果作了相关分析。结果表明:碘-碘化钾法不适合于测定玉米花粉活力,氯化三苯基四氮唑(TTC)法及离体萌发法能有效测定玉米花粉活力,TTC法来测定花粉的活力最为简单、准确。
The pollen viability was determined by iodine - potassium iodide method, triphenyltetrazolium chloride (TTC) method and in vitro germination method. The pollen viability was analyzed with correlation analysis with field measurement. The results showed that iodine - potassium iodide method was not suitable for the determination of corn pollen viability. TTC method and in vitro germination method could effectively measure the pollen viability. TTC method was the simplest and accurate .