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We report the current-voltage (I-V) characteristics and electrical conductivity of individual template-synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) nanowires (190 ± 6 nm in diameter and σRT : 11.2±2Ω-1cm-1)over a wide temperature range from 300 to 10K. With lowering temperature, the Ⅰ- Ⅴ characteristics become nonlinear around 50 K, and a clear Coulomb gap-like structure appears in the differential conductance (dI/dV)spectra. The temperature dependence of the resistance below 70 K follows ln R ∝ T-1/2, which can be interpreted as Efros-Shklovskii hopping conduction in the presence of a Coulomb gap. In addition, the influences of measurement methods such as the applied bias voltage magnitude, the two-probe and four-probe techniques used in the resistance measurements are also reported and discussed.