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开发了一种纳米金探针结合基因芯片用于micro RNAs(mi RNAs)检测的高灵敏度便携式生物传感器.靶标mi RNAs特异性捕获探针固定在芯片上,通过与纳米金探针杂交进行检测,最后利用过氧化氢(H2O2)还原四氯金酸(HAu Cl4)来放大检测信号.利用单个纳米金探针结合基因芯片可以检测到10 pmol/L的靶标mi RNAs,测得mi R-126在胎牛血清中的回收率为81.5%~109.1%.用这种生物传感器检测肺癌组织样本总RNA中的mi R-126,结果与定量PCR结果具有一致性.利用双纳米金探针进一步提高了检测灵敏度,可以检测到1 fmol/L的mi R-125a-5p.整个分析时间不超过1 h,并且实验结果可以用肉眼观察.这个平台可以同时检测肺癌相关mi R-126和mi R-125a-5p,并且具有费用低、快速和便捷的优势,有望用于mi RNAs的超灵敏可视化检测.
A highly sensitive and portable biosensor for the detection of micro RNAs (miRNAs) was developed by using nano-gold probes in combination with microarrays.The target miRNAs specific capture probes were immobilized on the chip and detected by hybridization with nano-gold probes, Finally, H2O2 was used to amplify HAu Cl4 to amplify the detection signal.Using a single gold nanoprobe probe and gene chip, 10 pmol / L of target mi RNAs could be detected and mi R-126 was detected in The recoveries of fetal bovine serum were 81.5% -109.1% .The detection of mi R-126 in total RNA of lung cancer samples by this biosensor was consistent with the results of quantitative PCR.The double gold nanoparticle probes further increased The sensitivity of this assay allows the detection of mi R-125a-5p at 1 fmol / L. The overall analysis time does not exceed 1 h, and experimental results can be observed with the naked eye. This platform can simultaneously detect lung cancer-related mi R-126 and mi R-125a -5p, with the advantages of low cost, rapidity and convenience, is expected to be used for the ultra-sensitive visualization of mi RNAs.