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建立了测定贝芪口服液中黄芪甲甙含量的薄层扫描测定法。采用硅胶Ｇ－ＣＭＣＮａ薄层板，以氯仿－甲醇－水（６５：３５：１０）为展开剂、１０％硫酸乙醇为显色剂，测定波长（λ_s）与参比波长（λ_Ｒ）分别为６００、７００ｎｍ。黄芪甲甙的量在１～６μｇ与斑点面积呈线性关系，ｒ＝０．９９８８。方法平均回收率为９８．２６±２．７６％（ｎ＝６）．三批供试品中黄芪甲甙的含量为０．１２２～０．１８０ｍｇ／ｍｌ，方法精密度（ＲＳＤ）分别为３．１０％、３．７１％、３．７０（ｎ＝４）。
A thin-layer scanning assay was established to determine the content of astragaloside in beryllium-containing oral liquids. A silica gel G-CMCNa thin-layer plate with chloroform-methanol-water (65:35:10) as developing solvent and 10% sulfuric acid ethanol as color reagent was used. The measurement wavelength (λ_s) and the reference wavelength (λ_R) were 600, respectively. 700nm. The amount of Astragaloside IV was linear with a spot area of 1 to 6 μg, r=0.9988. The average recovery of the method was 98.26±2.76% (n=6). The contents of Astragaloside IV in the three batches of test articles ranged from 0.122 to 0.180 mg/ml, and the method precisions (RSDs) were 3.10%, 3.71%, and 3.70 (n=4), respectively.