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目的:研究基质金属蛋白酶-9(MMP-9)在胎膜组织上的表达情况与早产胎膜早破的相关性。方法:荧光定量PCR(SYBR Green法)检测MMP-9mRNA;Western blot法检测MMP-9蛋白表达量;结果:早产胎膜早破组与足月胎膜早破组相比较,MMP9不存在统计学差异(Z=-1.783,P=0.075);与自发性早产组比较,MMP9不存在统计学差异(Z=-0.157,P=0.875);而早产胎膜早破组与正常足月产组比较MMP9存在统计学差异(Z=-3.316,P=0.001)。早产胎膜早破组MMP-9蛋白表达高于足月产组,之间的差异具有统计学意义(t=-12.339,P<0.01)。结论:MMP-9与早产胎膜早破存在相关性,在其发病机制中有着重要作用。
Objective: To investigate the correlation between the expression of matrix metalloproteinase-9 (MMP-9) in fetal membranes and preterm premature rupture of membranes. Methods: MMP-9 mRNA was detected by SYBR Green assay. The expression of MMP-9 protein was detected by Western blot. Results: Compared with preterm premature rupture of membranes group, there was no statistical significance (Z = -1.783, P = 0.075). Compared with spontaneous preterm labor group, there was no significant difference in MMP9 (Z = -0.157, P = 0.875); while preterm premature rupture of membranes group and normal term group There was a statistically significant difference in MMP9 (Z = -3.316, P = 0.001). The protein expression of MMP-9 in preterm premature rupture of membranes was higher than that in full-term group, the difference was statistically significant (t = -12.339, P <0.01). Conclusion: MMP-9 is associated with premature rupture of membranes and plays an important role in its pathogenesis.